In vivo and in vitro initiation of sperm
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Pillai, Murali C. ;Yanagimachi, Ryuzo ;Cherr, Gary N.
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Article
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1994
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John Wiley and Sons
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English
⚖ 729 KB
## Abstract Pacific herring (__Clupea pallasi__) gametes were cryopreserved in a simple extender (15% dimethyl sulfoxide in herring Ringers). Sperm remained viable for over 7 months when kept at liquid nitrogen temperature. A rapid cooling rate by directly plunging into liquid nitrogen allowed a ma