𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Rheological properties of chromosomal and plasmid DNA during alkaline lysis reaction

✍ Scribed by L. A. S. Ciccolini; P. Ayazi Shamlou; N.J. Titchener-Hooker; J. M. Ward; P. Dunnill


Publisher
Springer
Year
1999
Tongue
English
Weight
136 KB
Volume
21
Category
Article
ISSN
1615-7605

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Identification and Eradication of a Dena
✍ Jon R. Sayers; Debbie Evans; James B. Thomson πŸ“‚ Article πŸ“… 1996 πŸ› Elsevier Science 🌐 English βš– 108 KB

tion and precipitation as originally described by Birn-Many plasmid isolation procedures use strongly alboim and Doly (6). Recent discussions on the BIONET kaline conditions in the initial stages to facilitate lynewsgroup have revealed that several groups as well sis of the host bacteria. We demonst

Extraction of Superior-Quality Plasmid D
✍ Ramakrishna Lakshmi; Vijaya Baskar; Udaykumar Ranga πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 161 KB

termination were smaller than the symbol designating the mean value of the determination. ## Results and Discussion We found that with our assay conditions, TCEP completely reduced the GSSG that was present within the samples to GSH. Furthermore, the presence of TCEP did not interfere with the la

Alkaline-cell lysis through in-line stat
✍ Saethawat Chamsart; Tanyawat Karnjanasorn πŸ“‚ Article πŸ“… 2006 πŸ› John Wiley and Sons 🌐 English βš– 471 KB πŸ‘ 2 views

## Abstract A state‐of‐the‐art in‐line static mixer reactor (ISMR) was invented to lyse __E. coli__ cells and neutralize the cell lysate continuously and efficiently for the extraction of plasmid DNA. It comprised two connected static dynamic mixers, each 0.01 m in diameter and 0.9 m in length, one

Detection of extrachromosomal circular D
✍ Subrata Sen; Sudha Rani; Emil J. Freireich; Roger Hewitt; Sanford A. Stass πŸ“‚ Article πŸ“… 1992 πŸ› John Wiley and Sons 🌐 English βš– 403 KB

## Abstract Extrachromosomal circular DNAs ranging in size from submicroscopic molecules of approximately 100 kb to cytogenetically resolvable structures of 1000+ kb called minute and double‐minute chromosomes have been shown to harbor amplified genes in primary tumor cells, tumor cell lines, and d