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Rhamnogalacturonase: a novel enzyme that degrades the hairy regions of pectins

✍ Scribed by Henk A. Schols; Carolien C.J.M. Geraeds; Marjo F. Searle-van Leeuwen; Felix J.M. Kormelink; Alfons G.J. Voragen

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 674 KB
Volume: 206
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(90)84010-r

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✦ Synopsis

A rhamnogalacturonase (RGase), that could degrade the modified hairy regions (MHR) prepared from apple cell walls, was isolated and purified from a technical preparation of Aspergillus aculeatus. The RGase cleaved galactopyranosyluronic-rhamnopyranosyl linkages. No activity was observed towards other cell-wall polysaccharides orp-nitrophenyl glycosides. The optimal conditions for RGase were pH 34 and 40-50". MHR was degraded by RGase, and methylation analysis and '"C-n.m.r. spectroscopy indicated the products to have a tetrasaccharide backbone of alternating rhamnose and galacturonic acid residues. Some oligomers had a galactose residue 4-linked to rhamnose. The potential value of this new enzyme is considered. * Hairy (Ramified) Regions of Pectins, Part II. For Part I, see ref. 1 I.

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