RFLP analysis of asymmetric somatic hybrids betweenSolanum tuberosumand irradiatedS. brevidens

✍ Scribed by Y. S. Xu; E. Pehu

• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 650 KB
• Volume: 86
• Category: Article
• ISSN: 0040-5752
• DOI: 10.1007/bf00222666

✦ Synopsis

The nuclear genome composition of five asymmetric somatic hybrids, obtained by fusion of leaf protoplasts from Solanum tuberosum and gamma-irradiated leaf protoplasts from S. brevidens, have been analyzed at the molecular level. An analysis of 21 loci using linkage group-specific restriction fragment length polymorphism (RFLP) was included in the study. All five hybrids contained a complete set of the loci studied from S. tuberosum. The degree of elimination of alleles from the irradiated S. brevidens donor genome ranged from 10-65% in the five asymmetric hybrids analyzed. The detection of incomplete chromosomes, as well as non-parental bands in Southern hybridizations with RFLP markers, revealed extensive chromosome rearrangements in the asymmetric hybrids.