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Rewiring vesicle transports to probe the mechanism of polarized transports in neurons

โœ Scribed by Takao Nakata; Nobutaka Hirokwa


Book ID
116778088
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
52 KB
Volume
68
Category
Article
ISSN
0168-0102

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A fluorescence method for the direct measurement of CI-transport in isolated tonoplast vesicles is described. This technique utilises the Cl--sensitive fluorescent compound, 6-methoxy-l-(3-sulfonatopropyl)quinolinium (SPQ). This is a water-soluble compound with excitation and emission wavelengths of