The use of a chloride-sensitive fluoresc
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Andrew J. Pope; Roger A. Leigh
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Article
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1988
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Springer-Verlag
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English
โ 980 KB
A fluorescence method for the direct measurement of CI-transport in isolated tonoplast vesicles is described. This technique utilises the Cl--sensitive fluorescent compound, 6-methoxy-l-(3-sulfonatopropyl)quinolinium (SPQ). This is a water-soluble compound with excitation and emission wavelengths of