Review NMR studies of RNA dynamics and structural plasticity using NMR residual dipolar couplings

✍ Scribed by Melissa Getz; Xiaoyan Sun; Anette Casiano-Negroni; Qi Zhang; Hashim M. Al-Hashimi

• Publisher: Wiley (John Wiley & Sons)
• Year: 2007
• Tongue: English
• Weight: 745 KB
• Volume: 86
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.20765

✦ Synopsis

Abstract

An increasing number of RNAs are being discovered that perform their functions by undergoing large changes in conformation in response to a variety of cellular signals, including recognition of proteins and small molecular targets, changes in temperature, and RNA synthesis itself. The measurement of NMR residual dipolar couplings (RDCs) in partially aligned systems is providing new insights into the structural plasticity of RNA through combined characterization of large‐amplitude collective helix motions and local flexibility in noncanonical regions over a wide window of biologically relevant timescales (This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at [email protected]