Review fluorescence correlation spectroscopy for probing the kinetics and mechanisms of DNA hairpin formation

✍ Scribed by Alan Van Orden; Jaemyeong Jung

• Publisher: Wiley (John Wiley & Sons)
• Year: 2008
• Tongue: English
• Weight: 815 KB
• Volume: 89
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.20826

✦ Synopsis

Abstract

This article reviews the application of fluorescence correlation spectroscopy (FCS) and related techniques to the study of nucleic acid hairpin conformational fluctuations in free aqueous solutions. Complimentary results obtained using laser‐induced temperature jump spectroscopy, single‐molecule fluorescence spectroscopy, optical trapping, and biophysical theory are also discussed. The studies cited reveal that DNA and RNA hairpin folding occurs by way of a complicated reaction mechanism involving long‐ and short‐lived reaction intermediates. Reactions occurring on the subnanoseconds to seconds time scale have been observed, pointing out the need for experimental techniques capable of probing a broad range of reaction times in the study of such complex, multistate reactions. © 2007 Wiley Periodicals, Inc. Biopolymers 89: 1–16, 2008.

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