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Reversible thermal unfolding of thermostable cytochrome c-552

โœ Scribed by Hiroshi Nojima; Koyu Hon-nami; Tairo Oshima; Haruhiko Noda


Book ID
118917977
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
532 KB
Volume
122
Category
Article
ISSN
0022-2836

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Recent hydrogen exchange experiments on native cytochrome c implicate a sequential unfolding pathway in contrast to a simple two-state process. We have studied the heat-induced unfolding of this protein by using spectroscopic measurements to detect changes in conformation and proteolytic enzyme dige