Reversible rearrangement of vimentin-type intermediate filaments in cultured human skin fibroblasts from patients with lysosomal storage diseases.
✍ Scribed by Ivleva Tatiana; Wiederschain German
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 802 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
ABSTRACT
Immunofluorescence microscopy shows that unlike cytoplasmic microtubules (MT), vimentin‐type intermediate filaments (IF) are collected into ring‐shaped structures in affected fibroblasts. The altered IF organization could be observed in monolayers of polarized fibroblasts in the prolonged stationary growth phase and after replating ‐ upon their initial spreading. Transition from a discoid to an extended cellular form is accompanied by centrifugal dislocation of ring‐shaped IF structures towards the cell's active edge with gradual restoration of the radial fibrillar vimentin network. Spreading of affected cells occurred more slowly than that of control fibroblasts.