Reversible Oxidative Aggregation Obstructs Specific Proteolytic Cleavage of Glutathione S-Transferase Fusion Proteins

High-Yield Expression and Purification o

High-Yield Expression and Purification of Recombinant Proteins in Bacteria: A Versatile Vector for Glutathione S-Transferase Fusion Proteins Containing Two Protease Cleavage Sites

✍ Bernd U. Sehgal; Rebecca Dunn; Linda Hicke; Hilary Arnold Godwin 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 96 KB

40 and 43%, respectively) , they correspond to very small standard deviations of 0.11 and 0.39 pg/ml, respectively. Additional studies demonstrated that the recovery of 0.13, 0.35, 0.62, 2.7, 24, and 260 pg/ml VEGF in 10% pooled mouse EDTA plasma was 100 -120%; the average recoveries of 2, 5, and 2