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Reversible immobilization of proteins with streptavidin affinity tags on a surface plasmon resonance biosensor chip

โœ Scribed by Yong-Jin Li; Li-Jun Bi; Xian-En Zhang; Ya-Feng Zhou; Ji-Bin Zhang; Yuan-Yuan Chen; Wei Li; Zhi-Ping Zhang


Book ID
105890416
Publisher
Springer
Year
2006
Tongue
English
Weight
171 KB
Volume
386
Category
Article
ISSN
1618-2650

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The use of short peptide affinity tag sequences has become commonplace for the expression and purification of recombinant proteins. Many of these tags are antibody epitopes and detection of tagged proteins via Western blots is straightforward. However, the most common affinity tag used at present fo