Reversible denaturation of carbonic anhydrase provides a method for its adsorptive immobilization
✍ Scribed by Fereshteh Azari; Mohsen Nemat-Gorgani
- Book ID
- 101241896
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 149 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Palmityl-substituted sepharose 4B has been used for adsorptive immobilization of heat-denatured carbonic anhydrase. The native form of this enzyme does not show any affinity for binding to this hydrophobic support. However, through the process of denaturationrenaturation performed by heating and subsequent cooling of an enzyme solution in the presence of the matrix, it was possible to obtain a catalytically active immobilized preparation, which was used successfully in continuous catalytic transformations.
It is suggested that this simple procedure may provide a convenient method of immobilization for proteins, which are not normally adsorbed on hydrophobic supports.