Reversed-phase liquid chromatographic separation of antiretroviral drugs on a monolithic column using ionic liquids as mobile phase additives

✍ Scribed by Ramisetti Nageswara Rao; Bondigalla Ramachandra; Rajavarapu Mastan Vali

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 292 KB
• Volume: 34
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201000723

✦ Synopsis

Abstract

The use of 3‐methylimidazolium cation‐based ionic liquids (ILs) was evaluated as mobile phase additives for separation of antiretroviral drugs on a monolithic column by RP‐HPLC. Separation of eight commonly used antiretroviral drugs was achieved on a Chromolith Flash, RP‐18e column (25×4.6 mm, porous material) using water (pH 4.0 adjusted with acetic acid)/methanol v/v as a mobile phase containing ILs in a gradient elution mode. The effects of concentrations of ILs on retention, resolution and peak shape were studied and a regression equation correlating the interactions between stationary phase and the ILs was established. The retention of all the drugs was decreased notably by using 1‐butyl‐3‐methylimidazolium tetrafluoroborate, while 1‐ethyl‐3‐methylimidazolium methylsulfate reduced gradient drift drastically when compared to triethylamine.