Reversed-phase ion-pair liquid chromatographic determination of chlorophacinone residues in animal tissues

✍ Scribed by K. Hunter

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 644 KB
• Volume: 299
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)97856-2

✦ Synopsis

A sensitive and specific high-performance liquid chromatographic method for the identification and quantitation of chlorophacinone in animal tissues has been developed. Residues were extracted with chloroform-acetone

(1: 1, v/v). Clean-up of extracts was accomplished with a combined gel permeation-adsorption chromatographic procedure using Bio-Beads SX-3 and incorporating an on-line Sep-Pak silica cartridge. Residues were determined by ion-pair liquid chromatography, with the tetrabutylammonium ion as counter-ion, using a fixed-wavelength UV detector at 280 nm or a diode array detector at 285 nm. Recoveries from spiked liver tissue were around 90% at levels from 0.05 to 1 mg kg-'. A detection limit of 0.001 mg kg-' could be achieved in animal tissues. The diode array detector confirmed identification by matching spectra for residues down to 0.1 mg kg-' and below this level by multi-wavelength monitoring.