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Reversed-phase high-performance liquid chromatographic system for the rapid, automated purification of oligonucleotides

โœ Scribed by U. Birsner; U. Gilles; P. Nielsen; G.K. McMaster


Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
399 KB
Volume
402
Category
Article
ISSN
1873-3778

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Synthetic oligodeoxyribonucleotides containing a high guanine content have a tendency to form intra- or intermolecular complexes in solution make HPLC purification difficult or sometimes impossible. We have developed a simple method that has enabled us to purify a series of highly guanine-rich and s