Reversed Phase High Performance Liquid Chromatographic Separation of Esterquats with Indirect Spectrophotometric Detection

✍ Scribed by Yongsoon Chung; Sanghyun Park

• Publisher: Elsevier Science
• Year: 1998
• Tongue: English
• Weight: 213 KB
• Volume: 60
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1998.1635

✦ Synopsis

The reversed phase high performance liquid chromatographic (RP-HPLC) elution behavior of the basic esters and esterquats is discussed with data from indirect spectrophotometric detection. Three kinds of basic esters were synthesized by esterification of triethanolamine (TEA) with palmitic acid of three mole ratios (1:1.35, 1:2.00, and 1:3.00). Esterquats were prepared from quaternization of the basic esters with dimethyl sulfate (DMS). As a result, it was found that capacity factor (kЈ) values of mono-, di-, and triester of the basic esters and esterquats were significantly increased and the elution order reversed as the percentage of 1-propanol (1-PrOH) in mobile phase is increased from 75% to 80%. The RP-HPLC separation and determination of the basic esters and esterquats were also accomplished. Tetraphenylphosphonium bromide (TPPBr) was used as the detection reagent.