Reversed phase high performance liquid chromatographic determination with photodiode-array detection of nitroaromatics from former ammunition plants in surface waters

✍ Scribed by Feltes, J. ;Levsen, K.

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 389 KB
• Volume: 12
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240120908

✦ Synopsis

Only a few investigators used reversed phase HPLC separation Samples containing nitroaromatics have been taken from brooks and ponds in former ammunition production areas in Hessia (FRG) and Lower Saxony (FRG). The compounds were extracted by solid/liquid partition using Amberlite XAD 21418 resins and elution with dichloromethane. The samples were analyzed by reversed phase high performance liquid chromatography and detected at their optimum wavelength with a photodiode-array detector. The resulting UV-spectra and the retention times were compared with those of reference materials to identify the unknown compounds.The recoveries ranged from 85-1 05 %The detection limit for the method was at about 50 ng/l depending on the compound while the detection limit for the detector ranged from 0.9-1.4 ng. Mononitrotoluenes, dinitrotoluenes, and 2,4,6-of nitroaromatics in water samples and detection with UVdetector at254nminmostcases -141 used a ternary mixture of water/methanol/acetonitrile for the separation of four explosives.

The detection limits determined ranged from 10 to 26 pg/I, depending on the compounds studied. Walsh et al. [15] used both UV-and differential refractometer detection and acetonitrilelwater a s mobile phase. Maskarmecet al.

[16] used adsorption of munitions components from water samples on Porapak resins and reversed phase HPLC with a binary mobile phase with electrochemical detection a s well as UV detection at 254 nm. In the former mode a detection limit of 1 pgil was achieved using a gold-mercury electrode.

trinitrotoluene, as well as nitrotoluidines, have been identified in concentrations ranging from 0.1 -20 pgA.