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Reverse transcriptase-polymerase chain reaction assay for Acetylcholinesterase mRNA in rat brain

✍ Scribed by Rammohan Rao; Stephen Brimijoin

Book ID
105020105
Publisher
Springer
Year
1995
Tongue
English
Weight
920 KB
Volume
20
Category
Article
ISSN
0364-3190

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Quantitative Reverse Transcriptase-Polym
Quantitative Reverse Transcriptase-Polymerase Chain Reaction of Glucose Transporter 1 mRNA Levels in Rat Brain Microvessels
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We investigated the usefulness of reverse transcriptase-polymerase chain reaction (RT-PCR) to quantify glucose transporter 1 (GLUT1) mRNA in cerebral microvessels. The technique was validated using an in vitro-transcribed RNA fragment (riboprobe) of partial \(3^{\prime}\) noncoding sequence of rat b