Determination of 5-fluorouracil in human
Determination of 5-fluorouracil in human plasma by a simple and sensitive reversed-phase HPLC method
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R.A. Coe; R.A. Earl; T.C. Johnson; J.W. Lee
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Article
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1996
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Elsevier Science
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English
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A simple and sensitive reversed-phase HPLC method with UV detection was developed and validated for the quantitation of 5-fluorouracil (5-FC) in human plasma. After acidification and salting out, 5-FU was extracted into ethyl acetate and back-extracted into a basic buffer. The extract was adjusted t