Reverse-phase high-performance liquid chromatographic separation and electrochemical detection of norepinephrine, dopamine, serotonin, and related major metabolites

✍ Scribed by E. Kempf; P. Mandel

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 656 KB
• Volume: 112
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90285-2

✦ Synopsis

A convenient method for the determination of biogenic amines and their metabolites in small samples of brain tissue weighing from 0.5 to 5 mg, based on reverse-phase chromatography, is described. The biogenic amines, norepinephrine, dopamine, and serotonin, and the metabolites normetanephrine and 3-methoxytyramine were separated by ion-pair chromatography on a PBondapak phenyl column with an aqueous eluant, while the metabolites 3,4dihydroxyphenylacetic acid, homovanillic acid, 3-methoxy-4-hydroxyphenylglycol, and 5-hydroxyindoleacetic acid were separated on a PBondapak Cl8 column with a methanol aqueous mobile phase. The sensitivity of the method is in the picogram range, from 20 to 100 pg, depending on the substances analyzed.