Rev-erbα gene expression in the mouse brain with special emphasis on its circadian profiles in the suprachiasmatic nucleus
✍ Scribed by Hiroyasu Onishi; Shun Yamaguchi; Kazuhiro Yagita; Yoshiki Ishida; Xin Dong; Hidehito Kimura; Zhang Jing; Hidefumi Ohara; Hitoshi Okamura
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 700 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Rev-erbalpha is an orphan nuclear receptor that constitutively suppresses gene transcription. In the present study, the expression of Rev-erbalpha was investigated in the mouse brain by in situ hybridization using antisense cRNA probe. Positive Rev-erbalpha mRNA signals were detected widely in the brain with the highest expression in the suprachiasmatic nucleus (SCN). In the constant dark condition, the circadian expression profiles of Rev-erbalpha m RNA in the SCN showed a peak at early daytime (CT4) and a trough at early night time (CT16). The environmental lighting condition (light-dark environmental condition and exposure in the subjective night) did not alter the expression profiles. These findings indicate that Rev-erbalpha gene is a transcription factor intimately related to the circadian clock in the SCN.