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Retransformation of a revertant cell line with the adenovirus E1 oncogenes and vanadate

✍ Scribed by Jean Dessureault; Joseph M. Weber

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
214 KB
Volume
43
Category
Article
ISSN
0730-2312

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✦ Synopsis

We have isolated a revertant cell line (G5) from an adenovirus transformed rat cell line (F4) which failed to express the integrated viral oncogenes. To determine whether the reversion mutation was acting in cis or trans the G5 cells were co-transfected with an El gene bearing expression plasmid and a neomycin phosphotransferase bearing plasmid. G418-resistant colonies were picked and shown to express the El proteins and to be tumorigenic. This re-transformation could be partially mimicked by treatment with vanadate, an inhibitor of phosphotyrosine phosphatases. These results show that the continued presence of the El proteins was required to maintain the transformed phenotype, and that the reversion mutation was a cis-acting event affecting directly the integrated El genes.

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