Retinoid and Rexinoid Signaling : Methods and Protocols

✍ Scribed by Swapan K. Ray

• Publisher: Springer New York;Humana
• Year: 2019
• Tongue: English
• Leaves: 280
• Series: Methods in Molecular Biology 2019
• Edition: 1st ed. 2019
• Category: Library

✦ Synopsis

This volume explores the latest synthetic procedures for producing receptor-specific retinoids and rexinoids, molecular biology methods, and new technologies to demonstrate the therapeutic activities of molecules. The chapters in this book cover topics such as lentiviral delivery of shRNA constructs into acute promyelocytic leukemia cells for ATRA induced differentiation and autophagy; methods to analyze RAR signaling in colorectal cancer cells; differentiation of primary myoblasts by using RXR agonist; methodology for analyzing effects of retinoid treatment on nervous system development and larval swimming behavior; and protocols for assessment of autophagic flux in ATRA treated 2D and 3D breast cancer cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Retinoid and Rexinoid Signaling: Methods and Protocols is a valuable resources for graduate students, postdoctoral fellows, and principal investigators who are interested in further exploring the signaling mechanisms of these molecules in their specific preclinical models.

✦ Table of Contents

Front Matter ....Pages i-xiii
Proteomics to Predict Loss of RXR-γ During Progression of Epithelial Ovarian Cancer (Rajkumar S. Kalra, Sharmila A. Bapat)....Pages 1-14
Targeting Non-Genomic Activity of Retinoic Acid Receptor-Gamma by Acacetin (Jie Liu, Jian-gang Huang, Jin-Zhang Zeng)....Pages 15-31
Lentiviral-Mediated shRNA Approaches: Applications in Cellular Differentiation and Autophagy (Nina Orfali, Jennie N. Jeyapalan, Corinne L. Woodcock, Tracey R. O’Donovan, Dalyia Benjamin, Mary Cahill et al.)....Pages 33-49
Ligand Design for Modulation of RXR Functions (Claudio Martínez, José A. Souto, Angel R. de Lera)....Pages 51-72
In Vitro Models to Study the Regulatory Roles of Retinoids in Angiogenesis (Ayman Al Haj Zen)....Pages 73-83
Analysis of Retinoic Acid Receptor Signaling in Colorectal Cancer (Masamichi Imajo)....Pages 85-93
Methods to Assess Activity and Potency of Rexinoids Using Rapid Luciferase-Based Assays: A Case Study with NEt-TMN (Peter W. Jurutka, Carl E. Wagner)....Pages 95-108
Methods to Generate an Array of Novel Rexinoids by SAR on a Potent Retinoid X Receptor Agonist: A Case Study with NEt-TMN (Carl E. Wagner, Peter W. Jurutka)....Pages 109-121
Promoting Primary Myoblast Differentiation Through Retinoid X Receptor Signaling (Jihong Chen, Qiao Li)....Pages 123-128
Mapping Retinoic Acid-Dependant 5mC Derivatives in Mouse Embryonic Fibroblasts (Haider M. Hassan, T. Michael Underhill, Joseph Torchia)....Pages 129-141
Hemodynamics-Based Strategy of Using Retinoic Acid Receptor and Retinoid X Receptor Agonists to Induce MicroRNA-10a and Inhibit Atherosclerotic Lesion (Ding-Yu Lee, Jeng-Jiann Chiu)....Pages 143-169
Evaluating the Role of RARβ Signaling on Cellular Metabolism in Melanoma Using the Seahorse XF Analyzer (Christina Dahl, Per Guldberg, Cecilie Abildgaard)....Pages 171-180
Highly Sensitive Quantitative Determination of Retinoic Acid Levels, Retinoic Acid Synthesis, and Catabolism in Embryonic Tissue Using a Reporter Cell-Based Method (Leo M. Y. Lee, Selina T. K. Tam, Peter J. McCaffery, Alisa S. W. Shum)....Pages 181-192
A Behavioral Assay to Study Effects of Retinoid Pharmacology on Nervous System Development in a Marine Annelid (M. Handberg-Thorsager, V. Ulman, P. Tomançak, D. Arendt, M. Schubert)....Pages 193-207
Retinoic Acid as a Modulator of Proximal-Distal Patterning and Branching Morphogenesis of the Avian Lung (Rute S. Moura)....Pages 209-224
Translation of Effects of Retinoids and Rexinoids: Extraction and Quality Assessment of RNA from Formalin-Fixed Tissues (Iván P. Uray, Loretta László)....Pages 225-236
Assessing Autophagy During Retinoid Treatment of Breast Cancer Cells (Sarah Parejo, Mario P. Tschan, Manuele G. Muraro, Enrico Garattini, Giulio C. Spagnoli, Anna M. Schläfli)....Pages 237-256
Nonradioactive and Radioactive Telomerase Assays for Detecting Diminished Telomerase Activity in Cancer Cells after Treatment with Retinoid (Swapan K. Ray)....Pages 257-273
Back Matter ....Pages 275-276

✦ Subjects

Life Sciences; Biochemistry, general; Cancer Research