Retinoic acid stimulates growth hormone synthesis in human somatotrophic adenoma cells: Characterization of its nuclear receptors
✍ Scribed by Jean Guibourdenche; Charlotte Djakouré; Dominique Porquet; Patrick Pagésy; Cécile Rochette-Egly; Françoise Peillon; Jacques Yuan Li; Danièle Evain-Brion
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 112 KB
- Volume
- 65
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
In order to gain a better understanding on the possible role of retinoic acid (RA) on human GH secretion, we have characterized the expression of its nuclear receptors in somatotropic adenoma cell extracts. By immunoblotting with rabbit polyclonal antibodies directed against RARa, b, and g and RXRa and b, we could only detect the presence of RARa and RXRa proteins. The predominant expression of RXRa was confirmed at the mRNA level by Northern and slot-blot analysis. We then investigated the effect of RA on GH synthesis in cell culture of adenomatous somatotrophs. In cultured cells, RA (1 µM) stimulated GH secretion, increased intracellular GH content and GH mRNA levels within 72 h, suggesting a modulation of GH synthesis by RA.