Retinoic acid and 1,25-dihydroxyvitamin D3 inhibit tenascin-C expression in rat glioma C6 cells

✍ Scribed by Manuel Alvarez-Dolado; José Manuel González-Sancho; Cristina Navarro-Yubero; Luis F. García-Fernández; Alberto Muñoz

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 203 KB
• Volume: 58
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/(sici)1097-4547(19991015)58:2<293::aid-jnr9>3.0.co;2-h

✦ Synopsis

Tenascin-C (Tn-C) is an extracellular matrix protein with growth-, invasive-, and angiogenesis-promoting activities. Tn-C is upregulated during wound healing, tumorigenesis, and other pathological conditions. Highly malignant gliomas with poor prognosis exhibit high levels of Tn-C expression. Here we demonstrate that Tn-C RNA expression in glioma C6 cells is inhibited in a dose-dependent manner by retinoic acid (RA) and 1,25-dihydroxyvitamin D 3 (1,25-D 3 ). No additive or synergistic effects were found. Inhibition is maximum 24 hr after RA or 1,25-D 3 treatment, prior to a delayed cytotoxic effect starting at day 4-5 of treatment, and correlates with a reduction in the synthesis of Tn-C protein. Tn-C expression is also inhibited, but to a lesser extent by prostaglandin D2 (PGD2). Furthermore, both RA and 1,25-D 3 , but not PGD2 abolish the induction of Tn-C by the tumor promoter 12-O-tetradecanoyl phorbol 13-acetate. The inhibition of Tn-C expression might be relevant for the anti-cancer activity of RA and 1,25-D 3 .