Retinoic acid alone and in combination with cytosine arabinoside induces differentiation of human myelomonocytic and monoblastic leukaemic cells

✍ Scribed by Dr. H. T. Hassan; J. K. H. Rees

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 464 KB
• Volume: 6
• Category: Article
• ISSN: 0278-0232
• DOI: 10.1002/hon.2900060107

✦ Synopsis

The effect of retinoic acid (RA) alone and in combination with cytosine arabinoside (Ara-C) on differentiation of fresh human myeloid leukaemic cells from patients with AML was studied. Cells from six patients: three with acute myelomonocytic leukaemia AMMoL and three with acute monoblastic leukaemia AMoL with a percentage of blasts >70, were treated in an in vitro primary suspension culture with retinoic acid (10-' M), cytosine arabinoside (100 ng/ml) or both in combination. Non-adherent mononuclear cclls were seeded at a concentration of 5 x lo5 cells/ml in RF'MI 1640culture medium supplementedwith 20 per cent fetal bovine serum and 10 per cent (PHA-LCM) phytohaemagglutinin leucocyte conditioned medium and incubated for 6 days at 37°C in a humidified incubator containing 5 per cent CO, in air. Morphological and functional differentiation into terminal mature elements was induced in all leukaemia cells of the six patients following exposure to the combination of both agents. These results suggest the potential usefulness of the combination of a differentiating agent (retinoic acid) and an antileukaemic drug (cytosine arabinoside) in the treatment of acute myeloid leukaemias: AMMoL and AMoL. This combination warrants a clinical trial.