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Retinoblastoma protein expressed in human non-Hodgkin's lymphoma cells generates resistance against radiation-induced apoptosis

✍ Scribed by Ishii, Hideshi; Igarashi, Tadahiko; Saito, Toshiyuki; Nakano, Takashi; Mori, Masahiko; Ohyama, Harumi; Miyamoto, Tadaaki; Saito, Yasushi; Oh, Hakumei


Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
138 KB
Volume
55
Category
Article
ISSN
0361-8609

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✦ Synopsis


Inactivation of the retinoblastoma susceptibility (Rb) gene has been observed in various cancers. Nevertheless, in several cancer cases, including non-Hodgkin's lymphoma, previous investigations showed that the Rb gene product (pRb) was abundantly expressed with varying degree. Here we report the THS-SP1.1 cell line isolated from non-Hodgkin's lymphoma. The THS-SP1.1 cells abundantly expressed pRb and showed resistance against radiation-induced apoptosis. Culture with the antisense phosphorothioate oligonucleotide complementary to the Rb gene augmented the apoptosis of THS-SP1.1 cells after radiation, whereas the control oligonucleotides did not. These data showed that pRb abundantly expressed in the lymphoma cells inhibited radiation-induced apoptosis. Am