Retention study of some cation-type compounds using bile acid sodium salts as ion pairing agents in liquid chromatography
✍ Scribed by Medeea Radulescu; Victor Voicu; Andrei Medvedovici; Victor David
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 574 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0269-3879
- DOI
- 10.1002/bmc.1531
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✦ Synopsis
Abstract
The possibility of forming ion‐pairs between bile acids (sodium taurocholate, sodium taurodeoxycholate and sodium taurochenodeoxycholate) and different compounds (pralidoxime, obidoxime and pyridostigmine) having a cationic character has been studied in reversed‐phase liquid chromatography (RP‐LC). This study can be useful in understanding the role of bile acids in the transport of ionic species through hydrophobic membrane. The present study focused on the influence of mobile phase composition on the retention parameters of chosen compounds (percentage of acetonitrile, pH of aqueous omponent or ionic strength). For constant concentration of bile acids in aqueous component of mobile phase the functional dependencies between the logarithm of the retention factor (k) and the methanol content in the mobile phase followed a binomial pattern (U‐shaped), with a minimum positioned within the interval 70–85% methanol. Copyright © 2010 John Wiley & Sons, Ltd.