Retention of transformant specific type III collagen in dibutyryl cAMP treated Kirsten sarcoma virus transformed BALB 3T3 cells and in a flat revertant
✍ Scribed by Ryu-Ichiro Hata; Beverly Peterkofsky
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 816 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
We previously reported that Kirsten sarcoma virus transformed BALB 3T3 (Ki-3T3) cell cultures contained mainly type I collagen and about 30% of another type designated by us as Y and which appears to be type I11 collagen, krl(III)13. Clones of BALB 3T3 which exhibited contactinhibition were found to contain mainly type I collagen [al(I)12a2, and about 25% of another type (X) which was composed of three a1 chains differing from those of type I11 (Hata, R. and B. Peterkofsky, '77 Proc. Nat. Acad. Sci. (U.S.A.), 74: 2933-2937). Since dibutyryl 3': 5' cyclic adenosine monophosphate (dbcAMP) increases collagen synthesis and alters other transformation specific properties of Ki-3T3 cells, we determined whether treatment of Ki-3T3 cells with this compound restored the normal collagen phenotype. We also analyzed the collagen of a revertant of Ki-3T3 which exhibits properties similar to those of the dbcAMP treated transformant. Procollagen labeled with radioactive proline was isolated from the medium or cells of cultures and was converted to collagen with pepsin; the collagen was analyzed by carboxymethyl cellulose (CMC) chromatography or gel electrophoresis under denaturing conditions. Ki-3T3 cells treated with 0.5 mM dbcAMP continued to accumulate type I11 collagen but there was a n increase in the number of a, chains eluting from CMC columns in the same position as a i (I) suggesting increased accumulation of type X collagen. Although the revertant was similar to dbcAMP treated