Retention of particulate adenosine triphosphate from Mediterranean deep-sea water by glass-fibre and membrane filters
✍ Scribed by J. Gostan; N. Mayer-Gostan; J. -C. Romano
- Book ID
- 104757232
- Publisher
- Springer-Verlag
- Year
- 1985
- Tongue
- English
- Weight
- 474 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0025-3162
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✦ Synopsis
When deep-water samples from the Mediterranean Sea were passed through glass-fibre filters (Whatman GF/F), the adenosine nucleotide concentration of the particulate material was exceptionally low. The concentration was higher when the particulate material was concentrated on SM cellulose-membrane filters with porosities of less than 1 ,urn. There would seem to be two reasons for this: (1) inhibition of the bioluminescent reaction; (2) an apparently lower retention of ATP by the glass-fibre filters. In order to compare results obtained with the two different types of filters, a correction factor of two can be used if measurements are made with an internal standard. This constant factor can only be applied to samples from depths greater than 300 m.