Retention characteristics of a β-cyclodextrin polymer-coated liquid chromatographic column

✍ Scribed by Tibor Cserhàti; Esther Forgács; András Ujházy

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 545 KB
• Volume: 279
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)85071-q

✦ Synopsis

A silica based, &cyclodextrin polymer-coated liquid chromatographic column was prepared and its retention characteristics were determined using 28 ring-substituted phenol derivatives as solutes and methanol-water mixtures as eluents. The column showed a good separation power; the log k' value decreased linearly with increasing methanol concentration. Stepwise regression analysis demonstrated that the lipophilicity does not determine the retention on the /kyclodextrin polymer. Accordingly, the retention mechanism deviates from that of traditional reversed-phase columns. The steric characteristics and to a lesser extent the electronic parameters of the solutes have the greatest impact on the retention, which means that the inclusion complex formation also prevails after polymerization, resulting in a modified retention behaviour of the complexed solutes.