Retention behaviour of a porous styrene-divinylbenzene copolymer in the separation of fatty acids and related compounds
✍ Scribed by Miki Uchida; Takenori Tanimura
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 445 KB
- Volume
- 138
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
Using a porous styrene-divinylbenne copolymer for the chromatogmphic separation of straightchain fatty acids, we found the following properties: (1) a linear relationship between the logarithm of the capacity factor and the number of methylene groups in the alkyl chain, (2) almost identical slopes of the lines for three groups of fatty acid aualogues and benzoic acid esters and (3) a linear relationship between the slope and the concentration of methanol in the mobile phase.
INTRODUCXION
Porous silica, either alone or layered on spherical glass beads, has been widely used in high-performance liquid chromatography and these materials, coated or chemically bonded with various liquid phases, are being investigated in order to clarify the operating conditions and the nature of selectivity1-2. However, neither the chemical bond nor the silica matrix is stable under all conditions of chromatographic separations, especially in alkaline media3.
Porous styrene-divinylbenzene copolymers, which have no functional groups attached on the surface, are expected to be stable under a wider range of conditions and have been used in separations that are similar to reversed-phase chromatographf-The elution behaviour has been studied with dissociable compounds and neutral benzene derivatives. Reports have been published relating retention volume to the number of carbon atoms in the solute, the effect of the acidity of the mobile phase and the dissociation constants of the soIutes5*6.
In this work, the elution behaviour of fatty acids in their undissooiated form has been studied and compared with the elution behaviour of benzoic acid esters. Well defined characteristics of the resin were found in the separation of these materials.