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Retention behavior of trans isomers of eicosapentaenoic and docosahexaenoic acid methyl esters on a polyethylene glycol stationary phase

✍ Scribed by Svein A. Mjøs

Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 376 KB
Volume: 110
Category: Article
ISSN: 1438-7697
DOI: 10.1002/ejlt.200700225

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✦ Synopsis

Abstract

A polyethylene glycol (PEG) stationary phase was evaluated for the separation of mono‐trans isomers of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) methyl esters. The resolution patterns were compared to patterns achieved with previously applied conditions on a cyanopropyl phase. There were no overlaps between all‐cis EPA/DHA and their mono‐trans isomers on the PEG phase. Because of overlap between 22:0 and 22:1 isomers, the PEG column is not a good choice for analyses of EPA trans isomers in crude fish oils. However, if the saturated and monounsaturated fatty acids are not present in significant amounts, PEG can be a better choice than cyanopropyl columns.

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