Retargeting of T-cell-receptor gamma/delta+ lymphocytes against tumor cells by bispecific monoclonal antibodies. Induction of cytolytic activity and lymphokine production
✍ Scribed by Silvano Ferrini; Ignazia Prigione; Serafina Mammoliti; Maria Ines Colnaghi; Sylvie Menard; Alessandro Moretta; Lorenzo Moretta
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- French
- Weight
- 307 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
We have recently selected MAbs specific for different molecular forms of the TCR gammddelta (expressed by distinct cell subsets), able to activate TCR gamma/delta+ cells. Two of these reagents (GI and Al3) were used for the construction of bispecific MAbs in conjunction with a MAb (Mov 19) directed to ovarian carcinoma cells, usin the hybrid hybridoma technique. The G I -derived bispecific MAb GM33.9 efficiently induced lysis of Movl9 ovarian carcinoma cell lines (IGROVI and SW626) by G I + clones in a 4-hr %r-release assay. On the other hand, it was ineffective when Movl9-target cells were used. Comparable results were obtained with the A13-derived AM18.4 bispecific MAb when A13+ clones were used as effector cells. Bispecific MAbs were also able to induce secretion of IL-2 and TNF-alpha by TCR gammddelta+ clones when Movl9+ target cells were present.