Several methods have been described for using the polymerase chain reaction (PCR) to isolate fragments of DNA for genome mapping. We have developed an approach for isolating discrete fragments by amplifying DNA with single oligonucleotides (10mers) with arbitrarily selected sequences. The method is
โฆ LIBER โฆ
Restriction mapping of yeast artificial chromosomes by UV-mediated partial digestion
โ Scribed by C. Nobile; A. Forabosco
- Publisher
- Springer-Verlag
- Year
- 1994
- Tongue
- English
- Weight
- 660 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0938-8990
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## Abstract We have previously demonstrated that the __RCK__ gene involved in t(11;14)(q23;q32) and the more centromeric __MLL/ALL1__ gene involved in t(4;11)(q21;q23) and t(11;19)(q23;p13) are localized on different adjacent Notl fragments by using pulsedโfield gel electrophoresis (PFGE) analysis