The nif cistrons indentified by complementation analysis in the preceding paper (Dixon et al., 1977) were mapped with respect to hisD and to each other other by P1 cotransduction and three-factor reciprocal crosses. The order obrained was hisD nifB nifA (nifL) nifF nifE nifK nifD nifH. Analysis of h
Restriction mapping of deletions in the nif region of the Klebsiella pneumoniae chromosome
β Scribed by Appelbaum, Edward R. ;Kramer, Richard A.
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 632 KB
- Volume
- 179
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
Chromosomal DNA restriction fragments carrying the nitrogen fixation (niJ) and his genes of Klebsiella pneumoniae were identified in hybridization experiments using a plasmid derived from pRD1 as a radioactive probe. Restriction mapping of 26 genetically characterized chromosomal nif deletions provided a map showing the physical location of nfgenes along the chromosome.
π SIMILAR VOLUMES
Some restriction endonuclease fragments of nif DNA, when carried on small multicopy plasmids, inhibited nif expression in Klebsiella pneumoniae. A study of this inhibitory effect revealed, (1) that overproduction of the nifL gene product inhibited transcription of two nif operons examined, nifJ and
The histidine utilization (hut) operons of Klebsiella aerogenes were cloned into pBR322. The hut genes are wholly contained on a 7.9 kilobase pair fragment bounded by HindIII restriction sites and expression of hut is independent of the orientation of the fragment with respect to pBR322. A restricti