Restriction fragment length polymorphism ofc4genes in mice withtchromosomes

✍ Scribed by Mladen Golubić; Felipe Figueroa; Mario Tosi; Jan Klein

• Publisher: Springer-Verlag
• Year: 1985
• Tongue: English
• Weight: 609 KB
• Volume: 21
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00375377

✦ Synopsis

Genomic DNA was isolated from 29 t strains and 4 congenic lines of mice, digested with restriction endonucleases, and hybridized with a probe representing the complement component 4 (C4) gene. All but one of the enzymes revealed restriction fragment length polymorphism in this sample of C4-related genes. Double digestion analysis suggested the presence of three C4 gene copies in some of the t chromosomes and two copies in others. The enzymes distinguished 16 different haplotypes among the 33 strains tested. Based on their restriction fragment length patterns, the t strains could be divided into four groups with strains in each group more closely related to each other with respect to their C4-region genes than strains belonging to different groups. At least three of these four groups represent different branches of the evolutionary tree constructed for the t chromosomes. The C4-related genes of the chromosomes are in strong linkage disequilibrium with the class II genes of the H-2 complex. Typing for the Ss and Slp allotypcs of C4 has revealed the presence of the Ss ~ phenotype in two t strains and of the Slp ~' phenotype in one strain.