Restriction fragment alleles of the rabbit IGHG genes with reference to the rabbit IGHGCH2 or e locus polymorphism

✍ Scribed by P. J. Esteves; P. C. Alves; N. Ferrand; W. Van Der Loo

• Book ID: 104457518
• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 542 KB
• Volume: 33
• Category: Article
• ISSN: 0268-9146
• DOI: 10.1046/j.1365-2052.2002.00877.x

✦ Synopsis

Among domesticated mammals, rabbit (Oryctolagus cuniculus) is the only species possessing not more than one subclass of immunoglobulin (IgG) antibodies. The rabbit IGHGCH2 or e locus presents two serologically defined alleles, the e14 and e15 allotypes, which are correlated with amino acid variation at the IgG CH2–CH3 interface. Genetic studies, while revealing the adaptive value of this polymorphism, have relied so far entirely upon allo‐antisera. Here we show how these alleles can be distinguished by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) methods. The proposed PCR–RFLP approach allows the monitoring of IGHG locus diversity in rabbit.