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Restriction endonuclease mapping of three plasmids from Bacillus thuringiensis var. israelensis

โœ Scribed by Clark, Burton D.; Boyle, Thomas M.; Cho-Yam, Chu; Dean, Donald H.


Book ID
122973162
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
248 KB
Volume
36
Category
Article
ISSN
0378-1119

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The gene (cytA) coding for the 27 kDa polypeptide of the Bacillus thuringiensis var. israelensis mosquito larvicidal delta-endotoxin, was cloned into a plasmid containing the T7 bacteriophage promoter. The plasmid was used to transform an Escherichia coli strain containing the T7 RNA polymerase gene