Restriction cleavage maps of the DNAs of Streptococcus pneumoniae bacteriophages containing protein covalently bound to their 5′ ends
✍ Scribed by López, Rubens ;Ronda, Concepción ;García, Pedro ;Escarmís, Cristina ;García, Ernesto
- Book ID
- 104723814
- Publisher
- Springer
- Year
- 1984
- Tongue
- English
- Weight
- 807 KB
- Volume
- 197
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
Several pneumococcal bacteriophages showing a morphology similar to that previously described for Cp-1 (Ronda et al. 1981) have been isolated and purified from throat samples taken from healthy children. Three of these phages (Cp-5, Cp-7 and Cp-9) have been studied in detail and compared to Cp-1. The four phages differed in several respects, e.g. size, structural polypeptides, restriction enzyme cleavage patterns, etc. The DNA of Cp-5, Cp-7 and Cp-9 showed protease-sensitive transfecting activity. This, together with the results obtained by electrophoretic analyses as well as by isotopic labelling of these DNAs with [gamma-32P]ATP and polynucleotide kinase indicated that all these new phages have a protein covalently linked to the 5' ends of their DNAs as in the case of Cp-1 (García et al. 1983). Restriction enzyme cleavage maps of Cp-1, Cp-5, Cp-7 and Cp-9 have been constructed.