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Restricted expression of membrane type 1-matrix metalloproteinase by myofibroblasts adjacent to human breast cancer cells

✍ Scribed by Christèle Bisson; Silvia Blacher; Myriam Polette; Jean-Frédéric Blanc; Florence Kebers; Joëlle Desreux; Bernard Tetu; Jean Rosenbaum; Jean-Michel Foidart; Philippe Birembaut; Agnès Noel

Publisher
John Wiley and Sons
Year
2003
Tongue
French
Weight
457 KB
Volume
105
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

The membrane type‐1 matrix metalloproteinase (MT1‐MMP), a protease originally identified in breast carcinoma, is characterized by its capacity to activate other MMPs (MMP‐2 and MMP‐13) and to degrade extracellular matrix. Our study was undertaken to localize and identify the MT1‐MMP expressing cells in human breast adenocarcinomas. A textural analysis of images obtained by immunohistochemistry and in situ hybridization showed precisely the co‐expression of alpha smooth muscle actin (αSM actin) and MT1‐MMP in myofibroblasts. MT1‐MMP expression is confined to myofibroblasts in close contact with tumor cells. In sharp contrast, the expression of MMP‐2 was more widely distributed in both αSM actin positive and negative cells close to and at distance from cancer cell clusters. Our in vitro observations are consistent with the higher level of MT1‐MMP expression and of MMP‐2 activation observed in αSM actin positive fibroblasts derived from breast tumors, as compared to normal breast fibroblasts. Collectively, these results implicate myofibroblasts as major producer of MT1‐MMP in breast cancer and emphasize the importance of stromal‐epithelial cell interactions in their progression. © 2003 Wiley‐Liss, Inc.

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