Restricted expression of a novel retinoic acid responsive gene during limb bud dorsoventral patterning and endochondral ossification
✍ Scribed by Chazaud, Claire ;Bouillet, Philippe ;Oulad-Abdelghani, Mustapha ;Dollé, Pascal
- Book ID
- 102660656
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 1023 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0192-253X
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✦ Synopsis
Using a differential substractive hybridization cloning procedure we have recently identified Stra6 as a novel retinoic acid-induced gene in murine P19 embryonal carcinoma cells. The putative amino acid sequence of Stra6
shows no similarity with previously characterised proteins. We report here the pattern of expression of Strod transcripts during mouse limb development as revealed by in situ hybridization. In 8.5-9.0 days post-coitum (dpc) embryos, Stra6 was expressed in the lateral plate mesenchyme prior to limb bud outgrowth. By 9.5 dpc, expression was restricted to the proximal and dorsal forelimb bud mesoderm. Over the next 2 gestational days, Sfru6 expression was specific of the dorsal mesoderm of the undifferentiated forelimb and hindlimb buds with the exception of their distal-most region or progress zone. A novel proximal-ventral expression domain appeared, however, by 11 .O-11.5 dpc. Sfru6 also remained expressed in the flank mesoderm. From 1 1 5 1 3.5 dpc, Strod expression was restricted to the superficial mesenchyme surrounding the chondrogenic blastemas, and progressively extended until the distal extremities of the limbs upon disappearance of the progress zone. Progressive restriction of Stra6 expression to perichondrium and developing muscles was seen at 13.5-1 4.5 dpc. Upon the initiation of endochondral ossification (1 5.5-1 6.5 dpc), Stra6 expression was limited to the area of perichondrium apposing cells of high metabolic and proliferative activity (the elongation zone). We suggest that Strad may play a role in early dorsoventral limb patterning and later in the control of endochondral ossification.