Responses to human rhinovirus in CD45 T cell subsets isolated from tonsil

✍ Scribed by Sunethra Wilamasundera; David R. Katz; Benjamin M. Chain

• Book ID: 101309125
• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 147 KB
• Volume: 28
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/(sici)1521-4141(199812)28:12<4374::aid-immu4374>3.0.co;2-p

✦ Synopsis

Isotypes of CD45 have been used extensively as markers of memory and naive populations of T cells in peripheral blood. In this study, T cells were isolated from human tonsil and their proliferative response against human rhinovirus was measured. Unexpectedly, equivalent responses were found among the CD4 + CD45RA + and CD4 + CD45RO + populations of T cells. This response requires MHC class II-positive antigen-presenting cells. The time course of the T cell response in vitro was that of a classical recall response, and no proliferative response to the virus could be detected in human cord blood. These results suggest that tonsils contain a significant population of CD45RA + memory cells. The presence of this population may reflect ongoing stimulation with this common infectious agent, and the anatomical location of the T cells within the major lymphoid organ draining the naso-pharyngeal epithelial surface.