Response of methanogen populations to organic load increase during anaerobic digestion of olive mill wastewater
✍ Scribed by Aurora Rizzi; Mauro Zucchi; Sara Borin; Massimo Marzorati; Claudia Sorlini; Daniele Daffonchio
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2006
- Tongue
- English
- Weight
- 228 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0268-2575
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✦ Synopsis
Abstract
Process performances of an upflow anaerobic filter treating olive mill wastewater and the response of methanogenic Archaea to increasing volumetric organic load (VOL) were studied. At a VOL of 15 g chemical oxygen demand (COD) L^−1^ day^−1^, 90% of the influent COD was removed. Following a VOL increase from 6 to 15 g COD L^−1^ day^−1^, the polymerase chain reaction (PCR) titre of hydrogenotrophic Methanobacterium, determined by magnetic capture of the target DNA and group‐specific PCR based on the 16S rRNA gene, decreased from 10^11^ to 10^8^ cells g^−1^ sludge, while that of Methanomicrobiaceae and relatives increased from 10^4^ to 10^6^ cells g^−1^ sludge. Methanosaeta‐like acetoclastic methanogens were less affected by VOL variation and dominated at high VOL with a 16S rRNA gene PCR titre of 10^9^ cells g^−1^ sludge. Single‐strand conformation polymorphism analysis of the PCR‐amplified archaeal 16S rRNA gene showed a stable band pattern, indicating that VOL variation affected the methanogen PCR titre but not the archaeal community structure. Copyright © 2006 Society of Chemical Industry