## Abstract The metabolic burden and the stress load resulting from temperature‐induced production of human basic fibroblast growth factor is connected to an increase in the respiratory activity of recombinant __Escherichia coli__, thereby reducing the biomass yield. To study the underlying changes
✦ LIBER ✦
Response of fluxome and metabolome to temperature-induced recombinant protein synthesis in Escherichia coli
✍ Scribed by Christoph Wittmann; Jan Weber; Eriola Betiku; Jens Krömer; Daniela Böhm; Ursula Rinas
- Book ID
- 118472360
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 628 KB
- Volume
- 132
- Category
- Article
- ISSN
- 0168-1656
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## Abstract At the laboratory scale, sudden step increases from 30 to 42°C can be readily accomplished when expressing heterologous proteins in heat‐inducible systems. However, for large scale‐cultures only slow ramp‐type increases in temperature are possible due to heat transfer limitations, where