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Resonance Scattering Detection of Trace Hg2+ Using Aptamer-modified AuPd Nanoalloy Probe as Catalyst

✍ Scribed by Qingye Liu; Yanyan Fan; Aihui Liang; Zhiliang Jiang

Book ID
102097969
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
247 KB
Volume
29
Category
Article
ISSN
0256-7660

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✦ Synopsis

Abstract

The 5 nm AuPd nanoalloy in mole ratio of Au:Pd=32:1 was prepared, using sodium citrate as the stabilizing agent and NaBH~4~ as the reductant. The AuPd nanoalloy was modified by the aptamer to prepare an aptamer‐ AuPd (AptAuPd) probe for resonance scattering (RS) detection of 5.0–1250 nmol/L Hg^2+^. The AptAuPd‐Hg^2+^ aptamer reaction solution was filtrated by membrane, and the AptAuPd in the filtrate exhibited strong catalytic effect on the slow NiP particle reaction between NiCl~2~ and NaH~2~PO~2~, and the NiP particles showed a RS peak at 508 nm. The RS intensity decreased when Hg^2+^ concentration increased. The decreased RS intensity was linear to Hg^2+^ concentration in the range of 0.5–1250 nmol/L. The RS assays were used to determine Hg^2+^ in real samples, with good results.

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