Resonance Raman spectroscopy of metal-substituted bacteriochlorophylls: characterization of Raman bands sensitive to bacteriochlorin conformation
✍ Scribed by Arne Näveke; Karine Lapouge; James N. Sturgis; Gerhard Hartwich; Ingrid Simonin; Hugo Scheer; Bruno Robert
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 325 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0377-0486
No coin nor oath required. For personal study only.
✦ Synopsis
Fourier transform (FT) preresonance Raman spectra of Mg-containing bacteriochlorophyll a (BChla) and the metal-substituted Zn-, Ni-, Cu-and Co-bacteriopheophytins a (BPhea) allowed the characterization of those vibrational modes sensitive to the nature of the central metal, through its e †ect on the core size of these molecules. Six of the Raman modes active in the excitation conditions used (1064 nm, in preresonance with the transition of Q y the metal-bacteriopheophytin molecules) are shown to be sensitive to the conformation of the dihydrophorbin ring. Comparison of the FT-Raman spectra of BChla under conditions where the central Mg ion is either Ðve-or six-coordinate show that these modes may be used to distinguish modiÐcations of the dihydrophorbin core size as small as 1 pm, and thus that they may be used for characterizing conformational strains on BChla molecules both in vitro and in vivo.