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Resonance Raman quantification of the redox state of cytochromes b and cin-vivo and in-vitro

✍ Scribed by Minoru Kakita; Venkatesh Kaliaperumal; Hiro-o Hamaguchi

Publisher: John Wiley and Sons
Year: 2011
Tongue: English
Weight: 321 KB
Volume: 5
Category: Article
ISSN: 1864-063X
DOI: 10.1002/jbio.201100087

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✦ Synopsis

Abstract

We observe the redox state changes with respiration of cytochromes b and c in mitochondria in a living Saccharomyces cerevisiae cell as well as in isolated mitochondria with the very use of Raman microspectroscopy. The possibility of monitoring the respiration activity of mitochondria in vivo and in vitro by Raman microspectroscopic quantification of the cytochrome redox states is suggested. It will lead to a new means to assess mitochondrial respiration activity in vivo and in vitro without using any labelling or genetic manipulation. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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