Resolving power in a practical application of preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis: Preparation of Eastern encephalitis virus proteins

✍ Scribed by Stephen C. Marker; Paul Melby; Peter B. Jahrling

• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 577 KB
• Volume: 82
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90180-4

✦ Synopsis

Preparative polyacrylamide gel electrophoresis (PAGE) was used to separate the three proteins of Eastern equine encephalitis virus. The effect of gel length and gel concentration on the separation of these proteins was examined systematically. A single trial of preparative PAGE and the relative mobilities from analytical gel electrophoresis of a protein mixture provided the parameters needed to predict the elution times and resolution of the mixture components at other gel concentrations and gel lengths. The practical use of two simple formulas to determine the elution times and resolution of protein mixture components was demonstrated.