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Resolving Conformational and Rotameric Exchange in Spin-Labeled Proteins Using Saturation Recovery EPR

✍ Scribed by Michael D. Bridges; Kálmán Hideg; Wayne L. Hubbell


Book ID
106220607
Publisher
Springer Vienna
Year
2009
Tongue
English
Weight
723 KB
Volume
37
Category
Article
ISSN
0937-9347

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## Abstract EPR spectroscopy is a technique that specifically detects unpaired electrons. EPR‐sensitive reporter groups (spin labels or spin probes) can be introduced into biological systems via site‐directed spin‐labeling (SDSL). The basic strategy of SDSL involves the introduction of a paramagnet